Simple device for enumeration and isolation of luminescent bacterial colonies.

Simple device for enumeration and isolation of luminescent bacterial colonies.

Improved technique for identification and enumeration of methanogenic bacterial colonies on roll tubes by epifluorescence microscopy.

Methanogenic fluorescent colonies can be clearly identified on roll tubes by using an epifluorescence microscope equipped with a x 2 objective. Methanogenic and nonmethanogenic colonies could be counted in roll tubes prepared from methanogenic enrichment cultures. Late-developing colonies appearing after 25 days of incubation were mainly methanogenic.

Validation of drop plate technique for bacterial enumeration by parametric and nonparametric tests

Drop plate technique has a priority and preference compared with the spread plate procedure, because of less time, quantity of media, effort requirement, little incubator space, and less labor intensive. The objective of this research was to compare the accuracy and fidelity of drop plate method vs. spread plate method by parametric and nonparametric statistical tests. For bacterial enumeration...

Use of luminescent Leptospira interrogans for enumeration in biological assays.

Rapid and reliable in vitro methods for the detection of pathogenic leptospires, such as Leptospira interrogans, are lacking. The present study investigated the use of luminescence to replace the existing enumeration techniques. Transposon TnSC189 was modified to incorporate the luxCDABE cassette from Photorhabdus luminescens and was used to construct luminescent Leptospira spp. There was a lin...

Validation of a Simple and Rapid Method for Assessment of Intracellular Bacterial Asparaginase

L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous method has been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...